| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Ft. Zumwalt West High School | A | 8:05.13 | Verified |
| 2 | Marquette High School | A | 8:07.27 | Verified |
| 3 | Hickman High School | A | 8:07.64 | Verified |
| 4 | Lafayette (Wildwood) High School | A | 8:07.94 | Verified |
| 5 | Kirkwood High School | A | 8:09.01 | Verified |
| 6 | Eureka High School | A | 8:13.02 | Verified |
| 7 | Rock Bridge High School | A | 8:14.65 | Verified |
| 8 | Timberland High School | A | 8:31.40 | Verified |
