| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Lafayette (Wildwood) High School | A | 52.37 | Verified |
| 2 | Kirkwood High School | A | 51.98 | Verified |
| 3 | Hickman High School | A | 50.33 | Verified |
| 4 | Rock Bridge High School | A | 49.79 | Verified |
| 5 | Eureka High School | A | 50.88 | Verified |
| 6 | Ft. Zumwalt South High School | A | 51.62 | Verified |
| 7 | Troy Buchanan High School | A | 52.26 | Verified |
| 8 | Marquette High School | A | 52.38 | Verified |
