| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BROWN, Deion | SO | Kirkwood High School | 42.11 | Verified |
| 2 | EDMONDS, Elijah | SR | Ft. Zumwalt South High School | 41.70 | Verified |
| 3 | MAGEE, Ethan | SO | Battle High School | 39.56 | Verified |
| 4 | NELSON JR., Edward | SR | Hickman High School | 38.95 | Verified |
| 5 | KAZMAIER, Bryce | SR | Francis Howell High School | 38.96 | Verified |
| 6 | HARPER, Xavier | SR | Parkway South High School | 40.23 | Verified |
| 7 | CORTALEZZI, Sebastian | SR | Hickman High School | 41.83 | Verified |
| 8 | BROWN, Derrick | JR | Marquette High School | 42.94 | Verified |
