| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LITTRELL, Cale | SR | Hickman High School | 4:19.86 | Verified |
| 2 | FREINER, Tyler | JR | Timberland High School | 4:21.11 | Verified |
| 3 | HAUSER, Matthew | SR | Rock Bridge High School | 4:22.87 | Verified |
| 4 | PEIMANN, Trevor | SR | Timberland High School | 4:24.51 | Verified |
| 5 | CIMMARUSTI, Nick | SR | Francis Howell High School | 4:30.76 | Verified |
| 6 | KOOP, Ethan | SR | Marquette High School | 4:32.87 | Verified |
| 7 | MALEK, Nikolas | JR | Lafayette (Wildwood) High School | 4:33.08 | Verified |
| 8 | DEL CARMEN, Connor | SR | Marquette High School | 4:37.25 | Verified |
