| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BURKE, Stone | SR | Marquette High School | 1:58.82 | Verified |
| 2 | ARBANAS, William | SR | Kirkwood High School | 1:58.89 | Verified |
| 3 | HAUSER, Matthew | SR | Rock Bridge High School | 1:58.90 | Verified |
| 4 | COPELIN, Jaxson | SO | Ft. Zumwalt West High School | 1:59.09 | Verified |
| 5 | LITTRELL, Cale | SR | Hickman High School | 2:00.03 | Verified |
| 6 | PARKER, Nicholas | SR | Ft. Zumwalt West High School | 2:00.73 | Verified |
| 7 | DIEHL, Luke | SR | Parkway South High School | 2:02.19 | Verified |
| 8 | BLISS, Ethan | JR | Washington High School | 2:02.68 | Verified |
