| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BURNETT, Nia | JR | Ft. Zumwalt South High School | 27.81 | Verified |
| 2 | ANYE, Sarah | SO | Battle High School | 27.48 | Verified |
| 3 | WILKES, Mariah | 9 | Kirkwood High School | 27.31 | Verified |
| 4 | PORTER, Zakiya | JR | Rock Bridge High School | 25.82 | Verified |
| 5 | PARKER, Emma | 9 | Eureka High School | 27.26 | Verified |
| 6 | DONABY, Iyonna | SR | Hickman High School | 27.41 | Verified |
| 7 | DAVIS, SAMARA | 9 | Marquette High School | 27.78 | Verified |
| 8 | HALTER, Paige | JR | Lafayette (Wildwood) High School | 27.94 | Verified |
