| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GREEN, Leilani | 9 | Liberty (Wentzville) High School | 13.42 | Verified |
| 2 | BARROW, Eboni | SR | Marquette High School | 13.35 | Verified |
| 3 | HYDE, Kenyae | JR | Hickman High School | 13.05 | Verified |
| 4 | PORTER, Zakiya | JR | Rock Bridge High School | 12.57 | Verified |
| 5 | SCULLY, Mackenzie | JR | Kirkwood High School | 13.20 | Verified |
| 6 | NUNNELLY, Aneisha | SO | Rock Bridge High School | 13.25 | Verified |
| 7 | LIGHTBODY, Ilencia | SR | Timberland High School | 13.41 | Verified |
| 8 | NELSON, Marley | 9 | Liberty (Wentzville) High School | 13.48 | Verified |
