| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HOGAN, Cooper | JR | Troy Buchanan High School | 23.13 | Verified |
| 2 | BREDENKAMP, Ayden | JR | Ft. Zumwalt North High School | 22.82 | Verified |
| 3 | FAUPEL, Anthony | 9 | Francis Howell High School | 22.44 | Verified |
| 4 | WOODS, Cameron | SR | Christian Brothers College High | 22.41 | Verified |
| 5 | HOOD, Casey | SO | Rock Bridge High School | 22.42 | Verified |
| 6 | RICHARD, David | JR | Liberty (Wentzville) High School | 22.61 | Verified |
| 7 | BOEHLEIN, Timothy | SR | Washington High School | 23.06 | Verified |
| 8 | SCHAEFER, Gavin | JR | Timberland High School | 23.57 | Verified |
