| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | RAY, Lyriq | JR | Hazelwood Central High School | 49.15 | Verified |
| 3 | WALLACE, hannah | 9 | Cardinal Ritter High School | 48.46 | Verified |
| 4 | ANDERSON, Maya | SO | MICDS | 46.11 | Verified |
| 5 | HALSTEAD, Meghan | SR | Lindbergh High School | 47.92 | Verified |
| 6 | BELLAMY, Alexis | SR | MICDS | 48.54 | Verified |
| 7 | METZGER, Gracie | SO | Jackson High School | 51.69 | Verified |
| 8 | BEIDLE, Eva | JR | Oakville High School | 52.51 | Verified |
