| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | RAY, Julia | SO | MICDS | 5:12.02 | Verified |
| 2 | FLIEGE, Layla | JR | Jackson High School | 5:15.34 | Verified |
| 3 | GOLDFARB, Kylie | SR | John Burroughs High School | 5:22.69 | Verified |
| 4 | MARCUM, Isabella | SO | Fox High School | 5:24.29 | Verified |
| 5 | ANTHONIES, Courtney | JR | Seckman High School | 5:30.92 | Verified |
| 6 | PARISI, MARIA | 9 | Oakville High School | 5:30.99 | Verified |
| 7 | SPETNAGEL, Corinne | JR | John Burroughs High School | 5:31.21 | Verified |
| 8 | NOONAN, Riley | JR | MICDS | 5:34.00 | Verified |
