| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Webster Groves High School | A | Garrison | Murdock | Welch | Lemley | 4:16.45 | Verified |
| 2 | MICDS | A | Gira | Oliver | Goot | Anderson | 4:08.49 | Verified |
| 3 | John Burroughs High School | A | Wagner | Martyn | O'Brien | Goldfarb | 4:07.74 | Verified |
| 4 | Cardinal Ritter High School | A | brown | wallace | moody | Booker | 4:00.78 | Verified |
| 5 | Seckman High School | A | Ward | Smith | McLaughlin | Anthonies | 4:11.88 | Verified |
| 6 | Ladue Horton Watkins High School | A | Schulte | Huewe | Motes | White | 4:08.20 | Verified |
| 7 | Northwest (Cedar Hill) High Scho | A | Surratt | Wheeler | Bertani | Brown | 4:15.48 | Verified |
| 8 | Lindbergh High School | A | Halstead | Erpelding | Forbes | Forbes | 4:17.58 | Verified |
