| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Webster Groves High School | A | Gray | Lemley | Bratcher | Murdock | 1:52.38 | Verified |
| 2 | John Burroughs High School | A | Williams | Martyn | Harris | Mays | 1:50.86 | Verified |
| 3 | Ladue Horton Watkins High School | A | Ademuyiwa | Motes | Smith | White | 1:46.45 | Verified |
| 4 | Cardinal Ritter High School | A | taylor | brady | moody | brown | 1:42.95 | Verified |
| 5 | Poplar Bluff High School | A | Rowland | Anders | McCain | Cheek | 1:48.10 | Verified |
| 6 | Hazelwood Central High School | A | Futrell | Mapp | Mckenzie | Ray | 1:49.22 | Verified |
| 7 | Seckman High School | A | Lindsey | Ward | Larkin | McLaughlin | 1:50.61 | Verified |
| 8 | Oakville High School | A | Minor | Elza | Witte | Beidle | 1:54.47 | Verified |
