| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ladue Horton Watkins High School | A | Ademuyiwa | Smith | Whitley | White | 52.20 | Verified |
| 2 | Poplar Bluff High School | A | Rowland | Anders | McCain | Cheek | 51.89 | Verified |
| 3 | John Burroughs High School | A | Martyn | Mays | Harris | Elliott | 50.73 | Verified |
| 4 | Cardinal Ritter High School | A | taylor | brady | Taylor | Washington | 49.14 | Verified |
| 5 | Jackson High School | A | Bauer | Boyles | Evans | Kilhafner | 51.59 | Verified |
| 6 | Hazelwood Central High School | A | Ray | Mckenzie | Mapp | Stiles | 51.47 | Verified |
| 7 | Lindbergh High School | A | Halstead | Hertel | Hiers | Casaregola | 52.11 | Verified |
| 8 | Oakville High School | A | Minor | Elza | Beidle | Witte | 52.92 | Verified |
