| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WILKES, Keilah | JR | Pattonville High School | 2:19.65 | Verified |
| 2 | FLIEGE, Layla | JR | Jackson High School | 2:21.50 | Verified |
| 3 | GOLDFARB, Kylie | SR | John Burroughs High School | 2:21.93 | Verified |
| 4 | METZGER, Mallorie | SO | Jackson High School | 2:24.18 | Verified |
| 5 | WAGNER, Elizabeth | JR | John Burroughs High School | 2:24.35 | Verified |
| 6 | SCHULTE, Virginia | JR | Ladue Horton Watkins High School | 2:25.09 | Verified |
| 7 | SMITH, Alexandria | JR | Seckman High School | 2:26.17 | Verified |
| 8 | FORBES, Alyssa | SR | Lindbergh High School | 2:28.60 | Verified |
