| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LEMLEY, Margo | JR | Webster Groves High School | 1:02.52 | Verified |
| 2 | WARD, Angela | SO | Seckman High School | 1:01.48 | Verified |
| 3 | BOOKER, Kristen | SR | Cardinal Ritter High School | 1:00.32 | Verified |
| 4 | MOTES, Kayliah | JR | Ladue Horton Watkins High School | 59.05 | Verified |
| 5 | METZGER, Mallorie | SO | Jackson High School | 1:01.29 | Verified |
| 6 | HUBBARD, Camryn | SO | Pattonville High School | 1:00.54 | Verified |
| 7 | GIRA, Elizabeth | JR | MICDS | 1:01.96 | Verified |
| 8 | TROMPETER, Aleah | 9 | Fox High School | 1:02.85 | Verified |
