| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BRONNER, Jude | SO | Webster Groves High School | 53.59 | Verified |
| 2 | ROBINSON, Tyson | 9 | Ritenour High School | 52.35 | Verified |
| 3 | CLOUD, Raymond | 9 | DeSmet Jesuit High School | 51.99 | Verified |
| 4 | DAVIS, Elliott | SR | Ladue Horton Watkins High School | 50.22 | Verified |
| 5 | RUDANOVICH, Kaleb | SO | Seckman High School | 52.08 | Verified |
| 6 | MEEHAN, Joseph | JR | St. Louis University High School | 52.33 | Verified |
| 7 | LINGER, Ivan | SR | Lindbergh High School | 52.53 | Verified |
