| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | KILHAFNER, Ryleigh | JR | Jackson High School | 27.22 | Verified |
| 3 | BRADY, brooklyn | 9 | Cardinal Ritter High School | 26.58 | Verified |
| 4 | BROWN, aniyah | 9 | Cardinal Ritter High School | 25.21 | Verified |
| 5 | CHEEK, Emilee | JR | Poplar Bluff High School | 26.26 | Verified |
| 6 | BOYLES, Alexis | SR | Jackson High School | 27.00 | Verified |
| 7 | MAYS, Jillian | SR | John Burroughs High School | 27.33 | Verified |
| 8 | ADEMUYIWA, Zoe | JR | Ladue Horton Watkins High School | 27.62 | Verified |
