| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Timberland High School | A | Craft | Puszkar | Votruba | Moore | 9:55.89 | Verified |
| 2 | Rock Bridge High School | A | Parsons | Declue | Ford | Muskopf | 9:57.74 | Verified |
| 3 | Ft. Zumwalt South High School | A | Morrison | Humphrey | Hirsch | Gray | 10:07.96 | Verified |
| 4 | Ft. Zumwalt West High School | A | Linville | Maupin | Madrid | Lehmbeck | 10:19.29 | Verified |
| 5 | Hickman High School | A | Henderson | Lewandowski | Martin | McEuen | 11:00.61 | Verified |
| 6 | Battle High School | A | Bouchama | Hargrove | San Juan Rodriguez | Tatlow | 11:17.48 | Verified |
