| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Pattonville High School | A | Wilkes | Hautekeete | Hubbard | Brown | 9:54.18 | Verified |
| 2 | John Burroughs High School | A | Nichols | Wagner | Spetnagel | Goldfarb | 9:55.13 | Verified |
| 3 | MICDS | A | Gira | Oliver | Wyckoff | Ray | 10:25.59 | Verified |
| 4 | Ladue Horton Watkins High School | A | Chyu | Connors | Diwakaran | Hanley | 10:55.96 | Verified |
| 5 | Francis Howell Central High Scho | A | Bannon | White | Watkins | Pettig | 11:04.09 | Verified |
