| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Farmington High School | A | Wood | Earley | Sparr | Berkemeyer | 9:49.05 | Verified |
| 2 | Nerinx Hall High School | A | Schillinger | Medina | Jacknewitz | Crouch | 9:52.97 | Verified |
| 3 | Rockwood Summit High School | A | Brown | Pelchman | Rose | Thier | 9:56.01 | Verified |
| 4 | West Plains High School | A | Gunter | Kantola | Kantola | Woods | 10:21.75 | Verified |
| 5 | Cor Jesu Academy | A | Morie | Sise | Stirton | Luna | 10:27.74 | Verified |
| 6 | St. Joseph's Academy | A | Castellano | Guenther | Hughes | Kelly | 10:29.58 | Verified |
| 7 | DeSoto High School | A | Jones | PIGG | Petsch | Richardson | 10:38.35 | Verified |
| 8 | Festus High School | A | McDaniel | Faerber | Barnett | White | 10:44.97 | Verified |
