| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SCHULTE, Ava | 9 | Lutheran (St. Charles) High Scho | 1:06.70 | Verified |
| 2 | CASTILLO-ORELLANA, Maria | SO | Herculaneum High School | 1:06.07 | Verified |
| 3 | HOWARD, Brianna | SO | Normandy High School | 1:05.87 | Verified |
| 4 | JOHNSON, Sakoya | SR | Lutheran (St. Charles) High Scho | 1:01.46 | Verified |
| 5 | LACHANCE, Karlee | JR | Central (Park Hills) High School | 1:03.37 | Verified |
| 6 | CHRISTIAN, Maeta | JR | Metro High School | 1:05.91 | Verified |
| 7 | MOORE, Camila | SO | Herculaneum High School | 1:06.67 | Verified |
| 8 | STRUCKEL, Lindsey | 9 | Notre Dame (St. Louis) High Scho | NT | Unverified: |
