| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FISHER, Aaron | SO | Metro High School | 47.37 | Verified |
| 2 | SPROUS, Landon | FR | Potosi High School | 44.08 | Verified |
| 3 | PRITCHETT, Camden | SR | Kennett High School | 43.64 | Verified |
| 4 | BECK, Brison | JR | Lutheran (St. Charles) High Scho | 41.47 | Verified |
| 5 | BOCKENKAMP, Charles | SO | Central (Park Hills) High School | 42.14 | Verified |
| 6 | JEWELL, Dimitrous | JR | Orchard Farm High School | 44.01 | Verified |
| 7 | FULLER, Dallin | JR | Herculaneum High School | 44.14 | Verified |
| 8 | FISCHER, Nathan | SO | Orchard Farm High School | 48.03 | Verified |
