| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BROWN, Gabrielle | JR | Dexter High School | 5:30.82 | Verified |
| 2 | VANCE, Raven | SO | Herculaneum High School | 5:30.92 | Verified |
| 3 | STEWART, Clair | SO | Incarnate Word Academy | 5:39.50 | Verified |
| 4 | JACKSON, Isabelle | JR | Metro High School | 5:55.20 | Verified |
| 5 | SANSEGRAW, Celeste | SO | Potosi High School | 5:55.55 | Verified |
| 6 | SKILES, Alyson | 9 | Potosi High School | 5:59.29 | Verified |
| 7 | HUBBMAN, Louise | JR | Rosati-Kain High School | 6:18.43 | Verified |
| 8 | VANDEVENTER, Hope | SO | Lutheran (St. Charles) High Scho | 6:25.16 | Verified |
