| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TOAL, Elyssa | SR | Incarnate Word Academy | 2:25.15 | Verified |
| 2 | TOAL, Eden | SR | Incarnate Word Academy | 2:28.28 | Verified |
| 3 | BROWN, Gabrielle | JR | Dexter High School | 2:28.61 | Verified |
| 4 | PORTELL, Hallie | SR | Potosi High School | 2:29.21 | Verified |
| 5 | VANCE, Raven | SO | Herculaneum High School | 2:30.71 | Verified |
| 6 | HEEB, Lani | SO | Kennett High School | 2:35.68 | Verified |
| 7 | STECK, Anna | JR | Metro High School | 2:36.90 | Verified |
| 8 | SCHWIERJOHN, Lauryn | 9 | Orchard Farm High School | 2:40.28 | Verified |
