| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Rockwood Summit High School | A | Mcginnis | Turner | Rose | Wagner | 4:14.72 | Verified |
| 2 | Lutheran South High School | A | Saitz | Dicken | Karsten | Schelp | 4:13.98 | Verified |
| 3 | Hillsboro High School | A | Buettner | Buettner | Wright | VanHorn | 4:12.32 | Verified |
| 4 | Farmington High School | A | Wood | Gerstner | Earley | Berkemeyer | 4:07.62 | Verified |
| 5 | Nerinx Hall High School | A | Medina | Brady | Schillinger | Jacknewitz | 4:11.35 | Verified |
| 6 | DeSoto High School | A | Jones | Joyce | Mellor | Vogelsang | 4:12.68 | Verified |
| 7 | Notre Dame (Cape Girardeau) High | A | Brown | Smith | Givens | Jansen | 4:14.14 | Verified |
| 8 | West Plains High School | A | Brashers | Kantola | Hutchinson | Dixon | 4:22.34 | Verified |
