| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Windsor (Imperial) High School | A | Ealey | Winkelmann | Mancuso | Mancuso | 1:52.28 | Verified |
| 2 | Cor Jesu Academy | A | Varghese | Erusha | Graham | Wolf | 1:50.50 | Verified |
| 3 | Notre Dame (Cape Girardeau) High | A | Dohogne | Brown | Jansen | Givens | 1:48.50 | Verified |
| 4 | Rockwood Summit High School | A | Mcginnis | Jackson | Barafundi | Barafundi | 1:44.90 | Verified |
| 5 | DeSoto High School | A | Jones | Latham | Mellor | Mellor | 1:47.96 | Verified |
| 6 | Lutheran South High School | A | Allen | Hays | Karsten | Dicken | DQ | Verified |
| 7 | Festus High School | A | Batee | Bloodworth | Burkard | Biehle | 1:51.12 | Verified |
| 8 | Nerinx Hall High School | A | Johnson | Whatley-Blaine | Schirmer | Padberg | 1:55.20 | Verified |
