| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hillsboro High School | A | Windholz | Hildebrand | Mooney | Lopez | 47.46 | Verified |
| 2 | DeSoto High School | A | Punjani | Reissing | Pirtle | Radford | 44.94 | Verified |
| 3 | Central (Cape Girardeau) High Sc | A | Norfleet | Moore | King | Dixon | 44.16 | Verified |
| 4 | Sikeston High School | A | Green | Posey | Tilson | Lawrence | 43.95 | Verified |
| 5 | Cardinal Ritter High School | A | Moore | Tillman | Smith | Burks | 44.32 | Verified |
| 6 | Perryville High School | A | Garris | Jannin | Litterest | Rhyne | 44.58 | Verified |
| 7 | Rockwood Summit High School | A | Winkler | Bentrup | Wallace | Vu | 46.24 | Verified |
| 8 | Gateway High School | A | Washington | Kerney | Jones | Johnson | 49.40 | Verified |
