| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BUTCHKO, JOHN | SR | Rockwood Summit High School | 23.63 | Verified |
| 2 | NORFLEET, Kamarion | JR | Central (Cape Girardeau) High Sc | 23.59 | Verified |
| 3 | VALENCIA, Cruz | JR | Hillsboro High School | 23.37 | Verified |
| 4 | WRIGHT, Sam | SR | Hillsboro High School | 22.57 | Verified |
| 5 | DIXON, Keontae' | SO | Central (Cape Girardeau) High Sc | 22.95 | Verified |
| 6 | GARRIS, Aladdin | JR | Perryville High School | 23.55 | Verified |
| 7 | JOHNSON, Reece | SO | Festus High School | 23.61 | Verified |
| 8 | TILLMAN, Michael | SO | Cardinal Ritter High School | 23.65 | Verified |
