| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lift for Life Academy Charter Hi | A | Hall | Mills | Seals | Williams | 53.95 | Verified |
| 2 | Central (Park Hills) High School | A | Dortch | Johnson | Dailey | McGill | 53.41 | Verified |
| 3 | Kelly High School | A | Yount | Ray | King | Moore | 52.51 | Verified |
| 4 | Incarnate Word Academy | A | Moore | White | Moore | Flowers | 51.39 | Verified |
| 5 | Kennett High School | A | Parnell | Payne | New | Harrell | 51.48 | Verified |
| 6 | Miller Career Academy | A | Bruce | Fields | Flecher | Harris | 53.09 | Verified |
| 7 | Dexter High School | A | Turnbo | Haynes | Morgan | Pullen | 53.71 | Verified |
| 8 | Soldan International Studies Hig | A | evans | outlaw | Thomas-Reynolds | Davis | NT | Verified |
