| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hillsboro High School | A | Allison | Mooney | Mann | Valencia | 3:38.53 | Verified |
| 2 | DeSoto High School | A | Punjani | Reichmuth | Petty | Radford | 3:36.85 | Verified |
| 3 | Windsor (Imperial) High School | A | Mccrory | Julian | Ahrens | Chaney | 3:33.04 | Verified |
| 4 | West Plains High School | A | Brigman | Soulier | Zuchowski | Logue | 3:33.01 | Verified |
| 5 | Rockwood Summit High School | A | Emmenegger | BUTCHKO | Osterman | Krobath | 3:36.70 | Verified |
| 6 | Central (Cape Girardeau) High Sc | A | King | Dixon | Moore | Seabaugh | 3:35.03 | Verified |
| 7 | Cardinal Ritter High School | A | Smith | Stepps | Moore | Burks | 3:38.51 | Verified |
| 8 | Affton High School | A | Johnson | Johnson | Knapp | Minner | DNS | Verified |
