| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hillsboro High School | A | Windholz | Hildebrand | Suedkamp | Mooney | 1:37.45 | Verified |
| 2 | Festus High School | A | Roberts-Day | Branch | Martin | Johnson | 1:34.08 | Verified |
| 3 | Central (Cape Girardeau) High Sc | A | Norfleet | Moore | King | Dixon | 1:32.07 | Verified |
| 4 | West Plains High School | A | Brigman | Leazer | Lair | Miller | 1:30.93 | Verified |
| 5 | Cardinal Ritter High School | A | Tillman | Moore | Smith | Burks | 1:33.19 | Verified |
| 6 | DeSoto High School | A | Punjani | Pirtle | Reissing | Radford | 1:32.10 | Verified |
| 7 | Rockwood Summit High School | A | Winkler | Vu | Emmenegger | BUTCHKO | 1:35.68 | Verified |
| 8 | Gateway High School | A | Washington | Jimale | Kerney | Johnson | 1:46.67 | Verified |
