| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SIMPSON, Riley | SR | West Plains High School | 4:18.32 | Verified |
| 2 | SCHRAM, Ian | SO | Festus High School | 4:19.28 | Verified |
| 3 | KRIEG, Cullen | SO | Festus High School | 4:20.65 | Verified |
| 4 | AHRENS, Bradley | JR | Windsor (Imperial) High School | 4:27.62 | Verified |
| 5 | KNAPP, Gavin | SR | Affton High School | 4:47.80 | Verified |
| 6 | BRADSHAW, Sam | SR | Rockwood Summit High School | 4:53.97 | Verified |
| 7 | OSTERMAN, Aiden | 8 | Rockwood Summit High School | 4:56.68 | Verified |
| 8 | VAUGHN, Gavin | SO | Hillsboro High School | 5:00.80 | Verified |
