| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MELLOR, Meghan | SO | DeSoto High School | 27.56 | Verified |
| 2 | SAITZ, Elizabeth | SO | Lutheran South High School | 27.02 | Verified |
| 3 | HENDRICKS, Kristen | 9 | St. Joseph's Academy | 26.84 | Verified |
| 4 | BARAFUNDI, Benza | SR | Rockwood Summit High School | 26.57 | Verified |
| 5 | BIEHLE, Alexis | SO | Festus High School | 27.28 | Verified |
| 6 | BARAFUNDI, Christelle | 9 | Rockwood Summit High School | 27.01 | Verified |
| 7 | BOWERS, Katie | SO | West Plains High School | 27.36 | Verified |
| 8 | DORSEY, Te'mariah | SR | Central (Cape Girardeau) High Sc | 27.69 | Verified |
