| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Farmington High School | A | Currington | Mungle | Perry | Hunt | 8:18.99 | Verified |
| 2 | Festus High School | A | Krieg | Meyers | Wolk | Willis | 8:25.52 | Verified |
| 3 | West Plains High School | A | Simpson | Soulier | Stewart | Crites | 8:34.83 | Verified |
| 4 | Hillsboro High School | A | Allison | Gore | Allison | Mann | 8:37.21 | Verified |
| 5 | DeSoto High School | A | Shelton | Kaempfe | Schroeder | Voyles | 8:38.66 | Verified |
| 6 | Rockwood Summit High School | A | Bradshaw | Bordner | CARUSO | Osterman | 8:45.47 | Verified |
| 7 | Cardinal Ritter High School | A | Stepps | Jackson | Summers JR. | McField | 9:54.89 | Verified |
