| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CROUCH, Cami | JR | Nerinx Hall High School | 5:09.86 | Verified |
| 2 | SCHELP, Macy | SR | Lutheran South High School | 5:11.68 | Verified |
| 3 | LUNA, Francie | JR | Cor Jesu Academy | 5:20.03 | Verified |
| 4 | SMITH, Haley | SR | Notre Dame (Cape Girardeau) High | 5:21.12 | Verified |
| 5 | KANTOLA, Jordan | JR | West Plains High School | 5:24.49 | Verified |
| 6 | VOGELSANG, Kayla | JR | DeSoto High School | 5:26.13 | Verified |
| 7 | GUNTER, Jayna | JR | West Plains High School | 5:28.07 | Verified |
| 8 | PRINDIVILLE, Marie | SR | Nerinx Hall High School | 5:40.97 | Verified |
