| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | VANHORN, Kayla | JR | Hillsboro High School | 51.47 | Verified |
| 2 | CASSIMATIS, Carissa | SO | Farmington High School | 50.23 | Verified |
| 3 | DIXON, Laura | SO | West Plains High School | 50.16 | Verified |
| 4 | WRIGHT, Maya | JR | Hillsboro High School | 48.94 | Verified |
| 5 | HEATH, Jill | JR | DeSoto High School | 49.55 | Verified |
| 6 | DOCKERY, Taler | 9 | Central (Cape Girardeau) High Sc | 50.20 | Verified |
| 7 | KARSTEN, Katelynn | 9 | Lutheran South High School | 51.21 | Verified |
| 8 | VINCENT, Avery | JR | Rockwood Summit High School | 52.24 | Verified |
