| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ste. Genevieve High School | A | St. Clair | Wiegand | Bader | St. Clair | 8:28.43 | Verified |
| 2 | Dexter High School | A | Bell | Stone | Dorton | Cartwright | 8:33.23 | Verified |
| 3 | Potosi High School | A | Kanan | Cain | Griffin | Jarvis | 8:45.53 | Verified |
| 4 | Lutheran (St. Charles) High Scho | A | Fruend | Lind | McMullen | Hudson | 9:21.10 | Verified |
| 5 | Notre Dame (Cape Girardeau) High | A | Morris | Higgins | Scobey | Boyd | 9:28.16 | Verified |
| 6 | Metro High School | A | Gondzur | Appleton | Custis | Cade | 9:43.34 | Verified |
| 7 | St. Mary's (St. Louis) High Scho | A | Green | Ikeh | Leonard | Padberg | 9:49.13 | Verified |
| 8 | Orchard Farm High School | A | Knight | Reineke | VanDyke | Mobley | 10:14.33 | Verified |
