| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lift for Life Academy Charter Hi | A | Gillespie | Seals | Hardieway | Williams | 4:36.25 | Verified |
| 2 | Dexter High School | A | Turnbo | Neely | Pullen | Brown | 4:25.40 | Verified |
| 3 | Kelly High School | A | Yount | King | Stuckey | Moore | 4:20.96 | Verified |
| 4 | Ste. Genevieve High School | A | Wehner | Flieg | Reynolds | Aubuchon | 4:19.89 | Verified |
| 5 | Lutheran (St. Charles) High Scho | A | Johnson | Schulte | Raithel | Hudson | 4:23.14 | Verified |
| 6 | Incarnate Word Academy | A | Toal | Toal | Roberts | Moore | 4:24.12 | Verified |
| 7 | Herculaneum High School | A | Castillo-Orellana | Moore | Pinson | Vance | 4:26.70 | Verified |
| 8 | Metro High School | A | Kamara | Steck | Christian | Christian | 4:36.90 | Verified |
