| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ste. Genevieve High School | A | Wiegand | St. Clair | St. Clair | Bader | 3:44.88 | Verified |
| 2 | Orchard Farm High School | A | Jewell | Schwierjohn | Dulle | Bhat | 3:40.55 | Verified |
| 3 | Dexter High School | A | Farmer | Kyle | Dorton | Cartwright | 3:36.24 | Verified |
| 4 | Potosi High School | A | Mosier | Griffin | Brabham | Jarvis | 3:36.01 | Verified |
| 5 | St. Mary's (St. Louis) High Scho | A | Washington | Coleman | Casseus | Ikeh | 3:39.57 | Verified |
| 6 | Notre Dame (Cape Girardeau) High | A | Bruenderman | Garner | Taylor | Jansen | 3:40.31 | Verified |
| 7 | Lutheran (St. Charles) High Scho | A | Parr Jr. | Mclean | Gross | Lind | DNS | Verified |
| 8 | Lift for Life Academy Charter Hi | A | owens | Landers | Davie | Singleton | DQ | Verified |
