| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Potosi High School | A | Mosier | Hicks | Portell | Brabham | 1:40.03 | Verified |
| 2 | Miller Career Academy | A | Johnson | Slayton | Gleghorn | Williams | 1:37.22 | Verified |
| 3 | Lift for Life Academy Charter Hi | A | Johnson | owens | Singleton | Landers | 1:35.53 | Verified |
| 4 | Notre Dame (Cape Girardeau) High | A | Bruenderman | Garner | Taylor | Bruenderman | 1:31.83 | Verified |
| 5 | Orchard Farm High School | A | Williams | Bhat | Jewell | Pothoff | 1:34.47 | Verified |
| 6 | Soldan International Studies Hig | A | McElemurry | Afolhyan | Johnson | Edwards | 1:35.93 | Verified |
| 7 | Central (Park Hills) High School | A | Bockenkamp | Welch | Petty | Lotz | 1:37.53 | Verified |
| 8 | Ste. Genevieve High School | A | Irwin | Ogden | Schwent | Mcbride | DQ | Verified |
