| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KANTOLA, Brayden | JR | West Plains High School | 2:22.42 | Verified |
| 2 | MEDINA, Luciana | 9 | Nerinx Hall High School | 2:24.01 | Verified |
| 3 | SCHELP, Macy | SR | Lutheran South High School | 2:24.04 | Verified |
| 4 | JACKNEWITZ, Marisa | JR | Nerinx Hall High School | 2:24.67 | Verified |
| 5 | MELLOR, Erika | SR | DeSoto High School | 2:25.18 | Verified |
| 6 | SMITH, Haley | SR | Notre Dame (Cape Girardeau) High | 2:25.28 | Verified |
| 7 | SPARR, Alayna | JR | Farmington High School | 2:25.46 | Verified |
| 8 | PELCHMAN, Claire | JR | Rockwood Summit High School | 2:26.35 | Verified |
