| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LOFGREN, Alexa | SR | Affton High School | 1:04.56 | Verified |
| 2 | BRASHERS, Yasmine | JR | West Plains High School | 1:03.76 | Verified |
| 3 | WAGNER, Marketa | SR | Rockwood Summit High School | 1:01.59 | Verified |
| 4 | MELLOR, Erika | SR | DeSoto High School | 59.25 | Verified |
| 5 | NICHOLS, Lauren | 9 | Hillsboro High School | 1:01.30 | Verified |
| 6 | BERKEMEYER, Caroleigh | SR | Farmington High School | 1:01.67 | Verified |
| 7 | BISHOP, Tegan | SO | Perryville High School | 1:03.89 | Verified |
| 8 | BRADY, Lucy | JR | Nerinx Hall High School | 1:04.62 | Verified |
