| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Fair Grove High School | A | Ipock | Hall | Draper | Ghanem | 3:47.40 | Verified |
| 2 | Salem High School | A | Campbell | Mock | Coffman | Myslinski | 3:41.82 | Verified |
| 3 | Owensville High School | A | Breedlove | Whelan | Soest | Payne | 3:38.38 | Verified |
| 4 | St. James High School | A | Die | Redburn | Redburn | Altis | 3:37.47 | Verified |
| 5 | Buffalo High School | A | Bennett | Phillips | Casteel | Abraham | 3:38.20 | Verified |
| 6 | Osage High School | A | Hubkey | Watson | Childs | Vaughn | 3:41.52 | Verified |
| 7 | Mountain Grove High School | A | Walker | Norman | Norris | Glenn | 3:43.34 | Verified |
| 8 | Cuba High School | A | Chase | Dake | Hinojosa | Nelson | 4:03.62 | Verified |
