| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Osage High School | A | Claxton | Groll | Markovitz | Hubkey | 8:56.22 | Verified |
| 2 | St. Clair High School | A | Busse | Kern | Perkins | Tobben | 9:06.59 | Verified |
| 3 | Owensville High School | A | O'Neal | Zheng | Harris | Morgan | 9:08.52 | Verified |
| 4 | Ava High School | A | Miles | Mendel | Sallee | Goss | 9:19.00 | Verified |
| 5 | Buffalo High School | A | Glor | Sanderson | Walker | Walker | 9:19.79 | Verified |
| 6 | Willow Springs High School | A | Ortega | Perkins | Silva | Hicks | 9:20.00 | Verified |
| 7 | Mountain Grove High School | A | Boswell | Harker | Walker | Norris | 9:26.47 | Verified |
| 8 | Fair Grove High School | A | Lambeth | KIRKPATRICK | Buescher | Dennert | DQ | Verified |
| 9 | St. James High School | A | Deardeuff | Schultz | Chirico | Hughes | 9:54.80 | Verified |
| 10 | Cuba High School | A | Scott | Black | Brooks | Chase | DNS | Verified |
