Section 1 of 2
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Strafford High School | A | Keith | Clark | Means | Maples | NT | Verified |
| 4 | Ava High School | A | Gastineau | Croston | Gastineau | Mendel | 4:49.72 | Verified |
| 5 | Cuba High School | A | Black | Ijames | Moreland | Fulliam | 4:53.61 | Verified |
Section 2 of 2
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Owensville High School | A | Finn | Reynolds | Hendrix | Gehlert | 4:37.49 | Verified |
| 3 | St. Clair High School | A | Smith | Miller | Moore | Brand | 4:33.92 | Verified |
| 4 | Fair Grove High School | A | Mueller | Green | IPOCK | Luna | 4:27.29 | Verified |
| 5 | Osage High School | A | Bryant | Kessler | Johnson | Wolf | 4:30.63 | Verified |
| 6 | Mountain Grove High School | A | Sharp-MacPherson | Jarrett | Wake | Penner | 4:35.54 | Verified |
| 7 | St. James High School | A | Mullins | Crocker | Deardeuff | Bullock | 4:47.42 | Verified |
