| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Osage High School | A | Etherton | Moseley | Whyte | Walters | 2:02.54 | Verified |
| 2 | Ava High School | A | Gastineau | Fossett | Mendel | Akers | 1:59.38 | Verified |
| 3 | Willow Springs High School | A | Wilson | Mcnew | Mcdaniel | Scarbrough | 1:55.47 | Verified |
| 4 | Fair Grove High School | A | Mueller | GREEN | Tiller | Luna | 1:52.88 | Verified |
| 5 | Owensville High School | A | Angell | Branson | Candrl | Richardson | 1:54.31 | Verified |
| 6 | St. Clair High School | A | Smith | Mutschler | Brand | Moore | 1:56.30 | Verified |
| 7 | St. James High School | A | Hancock | Deardeuff | Mullins | Cahill | 1:58.50 | Verified |
