| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Cuba High School | A | Black | Moreland | Dong | Bast | 56.71 | Verified |
| 2 | Fair Grove High School | A | SUTHERLAND | GREEN | IPOCK | Mueller | 54.74 | Verified |
| 3 | Owensville High School | A | Angell | Richardson | Bruckerhoff | Watkins | 54.32 | Verified |
| 4 | Willow Springs High School | A | Mcdaniel | Scarbrough | Mcnew | Wilson | 53.29 | Verified |
| 5 | Strafford High School | A | Reed | Means | Crawford | Chadwell | 53.96 | Verified |
| 6 | St. James High School | A | Light | Satterfield | Crocker | Bullock | DNF | Verified |
| 7 | Osage High School | A | Bartlett | Adams | Kessler | Meisner | 56.41 | Verified |
| 8 | Ava High School | A | Pedersen | STANIFER | Gastineau | Akers | 59.04 | Verified |
