| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WOLF, Sara | SR | Osage High School | 5:16.76 | Verified |
| 2 | PENNER, Macey | JR | Mountain Grove High School | 5:26.68 | Verified |
| 3 | GIBSON, Mekayla | SR | Owensville High School | 5:34.82 | Verified |
| 4 | WAGGONER, Bethany | JR | Willow Springs High School | 5:46.07 | Verified |
| 5 | BRYANT, Juliana | JR | Osage High School | 5:50.27 | Verified |
| 6 | FULLIAM, Kaylee | 9 | Cuba High School | 5:53.69 | Verified |
| 7 | MELTON, Mallory | 9 | Ava High School | 6:00.00 | Verified |
| 8 | ABERCROMBIE, SADIE | SO | Buffalo High School | 6:08.51 | Verified |
| 9 | PERONA, Vanessa | 9 | St. James High School | 6:13.94 | Verified |
| 10 | KEITH, Piper | 9 | Strafford High School | 6:14.23 | Verified |
| 11 | SPOON, Hanna | SO | St. Clair High School | 6:15.92 | Verified |
| 12 | BRUTON, Rachel | 9 | Buffalo High School | 6:19.45 | Verified |
| 13 | WEIRICH, Kyah | JR | Owensville High School | 6:24.45 | Verified |
| 14 | MAPLES, Maddi | JR | Strafford High School | 6:27.08 | Verified |
| 15 | BROWN, Melayna | JR | Cuba High School | 6:28.45 | Verified |
| 16 | KINKEAD, Emily | JR | St. James High School | 7:08.05 | Verified |
| 17 | WILSON, Corbyn | 9 | Fair Grove High School | 7:48.90 | Verified |
| 19 | KILGORE, Karleyna | JR | Ava High School | NT | Unverified: |
