| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Palmyra High School | A | Janes | Reid | Markley | Snyder | 1:56.25 | Verified |
| 2 | Kirksville High School | A | Yardley | Novencido | Krepps | Lomela | 1:40.83 | Verified |
| 3 | Highland High School | A | Jones | Stutsman | Job | Mallett | 1:37.06 | Unverified: Knox County |
| 4 | Green City High School | A | Fields | Doosing | Barto | Peavler | 1:35.30 | Unverified: |
| 5 | Monroe City High School | A | Countryman | Martinez | Talton | Talton | 1:36.17 | Verified |
| 6 | Keokuk High School | A | Robinson | Mcwilliams | Glasgow | Potratz | 1:40.40 | Unverified: |
| 7 | Brookfield High School | A | Abongo | Boley | Starlin | Alexander | 1:41.18 | Verified |
| 8 | Clark County High School | A | Gregory | Poelvoorde | Plenge | Toolate | 1:58.00 | Unverified: Van Buren Relays |
