| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Scotland County High School | A | Small | HARVEY | ANDERS | Robinson | NT | Unverified: |
| 2 | Palmyra High School | A | Krigbaum | Sublette | Redd | White | 2:03.28 | Verified |
| 3 | Monroe City High School | A | Youngblood | Smith | Birt | Chinn | 1:59.09 | Unverified: Monroe City Invitational |
| 4 | Kirksville High School | A | Mccarty | Lyons | Herbst | Jackson | 1:56.20 | Verified |
| 5 | Highland High School | A | Abell | Bringer | Dreyer | Reed | 1:57.16 | Unverified: |
| 6 | Brookfield High School | A | Head | Daugherity | Thompson | Izard | 1:59.81 | Verified |
| 7 | Clark County High School | A | Brunk | Ross | St.Clair | Hess | NT | Unverified: |
